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Registros recuperados: 8
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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Scientia Agricola
Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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Determination of aspartate kinase activity in maize tissues Scientia Agricola
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Gratão,Priscila Lupino; Lea,Peter John; Azevedo,Ricardo Antunes.
Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Aspartate pathway; Maize; Lysine; Threonine; Methionine.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
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Phytoremediation: green technology for the clean up of toxic metals in the environment Braz. J. Plant Physiol.
Gratão,Priscila Lupino; Prasad,Majeti Narasimha Vara; Cardoso,Patrícia Felippe; Lea,Peter John; Azevedo,Ricardo Antunes.
The contamination of the environment by toxic metals poses a threat for "Man and biosphere", reducing agricultural productivity and damaging the health of the ecosystem. In developed nations, this problem is being addressed and solved to some extent by using "green technology" involving metal tolerant plants, to clean up the polluted soils. The use of naturally occurring metal tolerant plants and the application of genetic manipulation, should hasten the process of transferring this technology from laboratory to field. Therefore, it is essential to investigate and understand how plants are able to tolerate toxic metals and to identify which metabolic pathways and genes are involved in such a process. Recent advances in knowledge derived from the "omics",...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyperaccumulator plants; Phytoremediation; Toxic metals.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100005
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Potential of hydrogen (pH) differentially modulates cadmium stress response in abscisic acid-deficient sitiens tomato mutant Bragantia
Pompeu,Georgia Bertoni; Ambrosano,Guilherme Bovi; Vilhena,Milca Bartz; Carvalho,Rogério Falleiros; Gratão,Priscila Lupino; Andrino,Felipe; Lira,Simone Possedente de; Azevedo,Ricardo Antunes.
ABSTRACT Uptake of nutrients and cadmium (Cd) are dependent upon many factors, including plant species, ions concentration and pH. Tolerance to Cd-induced oxidative stress can be modulated by phytohormones such as abscisic acid (ABA), which induce the production of reactive oxygen species, activating proteins and enzymes involved in stress response and possibly stress tolerance. The present study aimed to evaluate the biochemical variations induced by Cd in ABA-deficient sitiens tomato mutant (sit) and its wild-type counterpart, Micro-Tom (MT), grown at different pH conditions. The plants were cultivated in nutrient solution (pH 5, 6 and 7; 20-days) and were then further grown over a 48-h period in 0 or 50 ?M CdCl2 at pH 6. Before Cd addition, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Micro-Tom; Mineral nutrition; Oxidative stress; Solanum lycopersicum.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000300317
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Response of Crotalaria juncea to nickel exposure Braz. J. Plant Physiol.
Cardoso,Patrícia Felippe; Gratão,Priscila Lupino; Gomes-Junior,Rui Alberto; Medici,Leonardo Oliveira; Azevedo,Ricardo Antunes.
The response of Crotalaria juncea seedlings to nickel (Ni) was investigated. Ni was shown to accumulate mainly in the root system, with little being translocated to the shoots. Catalase (CAT) and glutathione reductase (GR) responses to Ni were also analyzed. CAT activity did not exhibit a clear trend in response to Ni exposure, whereas GR activity appeared to respond to the stress induced by Ni. The results suggest that in C. juncea GR participates in the detoxification of Ni-induced reactive oxygen species via the glutathione-ascorbate cycle.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Heavy metals; Nickel; Reactive oxygen species.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200010
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Salt stress alleviation by seed priming with silicon in lettuce seedlings: an approach based on enhancing antioxidant responses Bragantia
Alves,Rita de Cássia; Nicolau,Mayara Cristina Malvas; Checchio,Mirela Vantini; Sousa Junior,Gilmar da Silveira; Oliveira,Francisco de Assis de; Prado,Renato Melo; Gratão,Priscila Lupino.
ABSTRACT Seed germination and seedling growth are the stages most sensitive to salt stress, which can induce ion-specific effects and oxidative stress. Although silicon (Si) has been known to improve plant defense systems against stressful-conditions, little is known about seed priming with Si and mechanisms of Si-mediated alleviation of salt stress in seedlings. To further the modulation of salt-stress responses and their relation with Si, seed priming with calcium silicate (0, 0.05 and 0.1 mM Ca2SiO4) was used in lettuce seeds to improve seedgermination and tolerance on subsequent salt-stress exposure (0 and 50 mM NaCl). The effect of Si-priming was assessed on germination, germination rate index and mean germination time and survival of seedlings in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Lactuca sativa; Oxidative damages; Salinity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052020000100019
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Simple procedure for nutrient analysis of coffee plant with energy dispersive X-ray fluorescence spectrometry (EDXRF) Scientia Agricola
Tezotto,Tiago; Favarin,José Laércio; Paula Neto,Ana; Gratão,Priscila Lupino; Azevedo,Ricardo Antunes; Mazzafera,Paulo.
Nutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and environmental samples by energy dispersive X-ray fluorescence spectrometry (EDXRF) was chosen as alternative technique to evaluate the simultaneous multielemental quantification of the most important essential elements in coffee (Coffea arabica L.) plants. Inductively coupled plasma atomic emission spectrometry and certified reference materials made from leaves were used to calibrate and check the trueness of EDXRF method for the determination of the concentration of several nutrients in coffee leaves and branches. Fluorescence spectrometry proved to be advantageous and presented low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; Metal toxicity; Mineral nutrition; Analytical procedure.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000400007
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The role of phytochromes in cadmium stress responses in tomato Bragantia
Gaion,Lucas Aparecido; Lorevice,Paulo Guilherme; Monteiro,Carolina Cristina; Gavassi,Marina Alves; D’Amico-Damião,Victor; Gratão,Priscila Lupino; Gasparino,Eduardo Custódio; Carvalho,Rogério Falleiros.
ABSTRACT It is well known that phytochromes mediate a wide range of photomorphogenic processes in plants. In addition, many studies have demonstrated the involvement of phytochromes as part of abiotic stress signaling responses. However, little is known about cadmium (Cd) stress regulation by phytochromes. Thus, in this study, we used the phyA (far red-insensitive; fri), phyB1 (temporary redinsensitive; tri) and phyB2 (phyB2) tomato (Solanum lycopersicum L.) mutants to investigate the roles of these three phytochromes on Cd stress responses. The plants were grown over a 21-d period in the presence of Cd. We evaluated plant growth, Cd and chlorophyll content and anatomical changes in the leaves. The results indicated that all genotypes were affected by Cd...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Heavy metal; Mutants; Plant anatomy; Solanum lycopersicum. L.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000100013
Registros recuperados: 8
Primeira ... 1 ... Última
 

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